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This paper describes the test results of low concentration butanol gasoline as fuel on motorcycle. It contains an immerse test to study material compatibility of 50% n-butanol gasoline(nB50) with some rubber, thermoplastics and Aluminum alloy usually used on motorcycle engine fuel system. An engine dyno test which is to compare the combustion characteristics of 20% n-butanol-gasoline (nB20) and gasoline. And an vehicle emission and fuel test which is to evaluate nB20 fuel emission characteristics and compliance of Taiwan motorcycles emission standards. The results shown there is no malfunction concern to use nB20 as fuel on the fuel injection motorcycle designed for gasoline. However, the NOx exhaust increase is a common issue of Alcohol alternative fuels on motorcycle.