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In order to control the mixture formation, a mixture injected 4-valve SI engine was developed with a small mixture chamber and mechanically driven mixture injection valve installed into the cylinder head. The mixture injection valve was located at the center of the combustion chamber. The mixture was injected from the final stage of the intake stroke to the beginning of the compression stroke. The mixture distribution and in-cylinder flow field inside the combustion chamber were measured by a pair of laser two-dimensional visualization techniques. A planar-laser-induced exciplex fluorescence technique was used to visualize the in-cylinder mixture formation by obtaining spectrally separated fluorescence images of liquid and vapor phase fuel distribution. Particle image velocimetry (PIV) was used to obtain flow field images. In the case of the mixture injected SI engine, the mixture injected into the swirl center was retained during the compression stroke.