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The inedible portion of plant biomass in closed regenerative life support systems must be reprocessed producing recyclable by-products such as carbon dioxide, sugars, and other useful organic species. High solids biomass slurries containing up to 27 wt% were successfully prepared in a stirred batch reactor and then pumped using a single piston valveless pump. Wheat straw, potato, and tomato crop residues were acid hydrolyzed using 1.2 wt% sulfuric acid at 180°C and 1.2 MPa for 0.75-1.5 hours. Viscosity for a 25 wt% acid hydrolyzed wheat straw emulsion (Bingham-plastic) was 6.5 centipoise at 3 cm/sec and 25°C.

The ability to aseptically remove samples and products, and the capability for addition of materials to sterile or otherwise microbially susceptible systems have always been compromised by the lack of a reliable means of sterilizing the mating fixtures. Cultures of mammalian cells are particularly vulnerable to microbial contamination due to the complexity of nutrient media and the lengthy periods required for cell growth. The Microwave Sterilizable Access Port has been developed to overcome this limitation. The system consists of three primary components: a microwave power source, a combined sterilization chamber/in-line valve port assembly, and a specimen transfer interface. Microwave energy is transmitted via coaxial cable to a small pressurized chamber that serves as a sterile transition between the surrounding environment and the system during transfer of materials.