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Effects of chemical reaction characteristics of premixed fuel were experimentally studied in a dual fuel compression ignition engine using port injection (PI) of gasoline-like component and direct injection (DI) of diesel fuel. Octane number of port injection fuels, direct injection timing and injection amount ratio between PI and DI were swept to assess the interaction between chemical reaction and mixture distribution in a combustion chamber. Chemical kinetic study using multi-zone modeling was also performed in order to explain experimental results under quiescent condition.