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The integration of membrane-aeration technology with biological water processors has direct application to wastewater treatment in microgravity because of the ability to diffuse gases across the membrane without two-phase interactions (gas-liquid). Membrane-aeration bioreactors have demonstrated the ability to deliver a terminal electron acceptor (O2) and substrates (CH4 and H2) to biofilms attached to the membrane surface. However, the process performance of these systems has been limited by mass transfer constraints. A novel bubbleless membrane-aeration bioreactor was design and tested at Kennedy Space Center. The Aerobic Rotational Membrane System (ARMS) consists of a rotational membrane module inside of a pressurized reactor vessel.

The purpose of this research is to determine the feasibility of a unique denitrifying composter to stabilize trash from space-habitation (STS, ISS, ALS) life support activities. Design criteria were derived from variables to be manipulated and those to be held constant. A pre-existing aerobic composter was used and engineering tests run to ensure that requirements were met. Key experimental variables were identified: NO3- concentration and rate of addition, O2 concentration, mixing duration and frequency, and inoculum. Independent variables were pH, temperature, moisture, C:N ratio, feed material, size reduction, feed addition rate, and mode of operation. Important performance parameters included: maximization of desired outcomes – BOD5 removal, CO2 production, waste stabilization, and denitrification – and minimization of undesired products – N2O, NH3, and volatile organic compounds.

The effluent, or ‘broth’ output of bioreactors fed Advanced Life Support (ALS) solid wastes contains suspended particulate material composed of undigested waste residues and microbial cells. ALS crop scientists have required the removal of particulates from these solutions before they can be used to recycle soluble crop nutrients, also contained in the effluents, to hydroponic crop growing systems. A two-stage filtration system was designed and evaluated with different membrane filtration elements. The first stage, prefiltration, was designed to remove large particles (μm to mm range). The key study factors for prefiltration were: (1) filter bag pore size – 50 vs. 5 vs. 0.5 μm- and (2) rinse solution - none, de-ionized water, and simulated graywater). Between 22 to 30% of the liquid was retained by the pre-filters and retained solids, indicating the necessity of introducing a rinsing step to better recover soluble crop nutrients.

Bioprocessing of human fecal wastes may be an important means for recycling of crop nutrients within a closed Advanced Life Support System. The objectives of this study were to determine the levels of key crop nutrients that can be extracted from human feces that had been autoclave sterilized vs. those that had not. When compared with inedible ALS grown wheat residues, the contribution of feces, which has an ash content 13% to the total potential, recoverable minerals may be small. This paper discusses results from bioreactor runs obtained using continuous stirred tank reactors with an 8 day batch culture of autoclaved or not autoclaved feces. The results suggest that feces should not be autoclaved if mineral recovery is desired. Biodegradation of feces ranged from 27 to 39% in 8 days, with 67 to 79% reduction in soluble total organic carbon (TOC) and concomitant production of carbon dioxide (CO2).

Bioreactor technology for bioprocessing graywater solutions in microgravity is under development by NASA at Johnson Space Center and at major aerospace companies. Inoculum sources have been inconsistent. Startup and subsequent operation of ground-based bioreactors may have been adversely affected by this inconsistency and/or by inoculation procedures. The goal of the research reported in this paper is to develop an inoculum that will completely biodegrade Igepon T42 soap to carbon dioxide and water under anaerobic, denitrifying conditions and with process conditions set by bioreactor design requirements for microgravity operation. Potential inoculum sources from two habitats within the KSC-ALS breadboard project were developed for potential use. The effects of pH (7.2 vs. 9.0, buffered) on soap degradation by the two inocula was determined in a flask study. Nearly all of the soap was degraded at pH 7.2 while nearly none was degraded at pH 9.0. Both inocula behaved similarly.

The KSC Breadboard Scale Aerobic Bioreactor (B-SAB) was used to bioprocess inedible wheat crop residues to provide recycled nutrients to support crop growth in the JSC Variable Pressure Growth Chamber (VPGC) as part of the 91 day JSC-Lunar/Mars Life Support Test Project Phase III. To meet the wheat nutrient demand at JSC, the KSCB-SAB was operated at both a higher loading rate (35 gdw L-1 compared with 20 gdw L-1) and at a slower retention time (21 days compared with 8 days) than we had used in previous bioreactor (continuous stirred tank reactor - CSTR) studies. The bioreactor operated for 19 weeks-8 weeks startup and steady state stabilization then 11 weeks of operation with the broth harvested weekly. Filtered broth was amended with nutrients and transported to JSC for integration into the VPGC wheat growth component of L/MLSTP Phase III. Biodegradation of JSC wheat residues was a constant 45% during steady state bioreactor operation, and similar to previous B-SAB runs.

Bioregenerative resource recovery components for Advanced Life Support systems will need to be reliable and stable for long duration space travel. Since 1989, bioregenerative life support research at the ALS Breadboard Project has examined processing of inedible crop residues in bioreactors for recovery of nutrients for replenishment of crop hydroponic solutions. Bioreactor operation has been reliable as demonstrated by continuous operation for up to 418 days with long periods of steady state conditions. Bioreactors have demonstrated stability following unplanned, non-lethal perturbations in pH, temperature, dissolved oxygen, and inedible residue supply. In each instance, a rapid return to steady state conditions was observed.

The National Aeronautics and Space Administration (NASA) intends to continue the human exploration of outer space. Long duration missions will require the development of reliable regenerative life support processes. The intent of this paper is to define the Kennedy Space Center Controlled Ecological Life Support System (CELSS) research plan for the development and testing of three candidate biological processors for a hybrid biological and physical-chemical waste recycling system. The system would be capable of reclaiming from inedible plant biomass, human metabolic waste, and gray water those components needed for plant growth (carbon dioxide, water, and inorganic salts), while eliminating noxious compounds and maximizing system closure. We will colaborate with AMES Research Center (ARC), Johnson Space Center (JSC), and academia, to design a functional biological-based waste processing system that could be integrated with the planned Human Rated Test Facility (HRTF) at JSC.

Bioregenerative processes for the replenishment of plant nutrients in CELSS are being evaluated. Continuous operation of a breadboard-scale aerobic bioreactor (breadboard-scale aerobic bioreactor, 120 L working volume) has been used successfully to resupply partially the nutrients required for hydroponically grown wheat (4 m2 growing area, 57 days bioreactor operation) and potato (8 m2 growing area, 310 days bioreactor operation). Bioreactor performance, measured by reduction in volatile solids (27 to 37% wheat, ca. 51% potato) and mineralization of biomass C (24 to 37% wheat, 35 to 61% potato), depended on process parameters such as retention time.

Three Intermediate-Scale Aerobic Bioreactors were designed, fabricated, and operated. They utilized mixed microbial communities to bio-degrade plant residues. The continuously stirred tank reactors operated at a working volume of 8 L, and the average oxygen mass transfer coefficient, kLa, was 0.01 s-1. Mixing time was 35 s. An experiment using inedible wheat residues, a replenishment rate of 0.125 day-1, and a solids loading rate of 20 gdw day-1 yielded a 48% reduction in biomass. Bioreactor effluent was successfully used to regenerate a wheat hydroponic nutrient solution. Over 80% of available potassium, calcium, and other minerals were recovered and recycled in the 76-day wheat growth experiment.

Biomass processing at the Kennedy Space Center CELSS breadboard project has focused on the evaluation of breadboard-scale enzymatic hydrolysis of wheat residue cellulose (25%, w/w). Five replicate runs of cellulase production by Trichoderma reesei (QM9414) and enzymatic hydrolysis of residue cellulose were completed. Enzymes were produced in 10 days (5 L, 25 g [dry weight] residue). Cellulose hydrolysis (12 L, 50 g [dry weight] residue) using these enzymes produced 5.5 to 6.0 g glucose liter-1 in 7 days. Cellulose conversion efficiency was 29%. These processes are feasible technically on a breadboard scale, but would only increase the edible wheat yield 10%.

The initial goal of the Controlled Ecological Life Support System (CELSS) Breadboard Project is to develop and evaluate a ground-based bioregenerative system scaled to support the equivalent of one crew member. The Biomass Production Chamber (BPC) is the plant growing module of this project. We describe here the characterization of the microbial constituents of the BPC during production tests of hydroponically-grown crops of wheat and soybeans. Bacterial and fungal viable counts were determined for the hydroponic solution, dehumidifier condensate water, and atmosphere. Bacterial communities were characterized by taxonomic identification (Vitek AutoMicrobic System) of randomly selected isolates. For all crop tests, bacteria dominated the microflora of both the hydroponic solution (range--104 to 106 colony forming units [cfu] per mL), and dehumidifier condensate (103 to 106 cfu/mL).