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Membranes are highly desirable for separating gases in life-support applications. They are small, light, efficient, selective and require little operational or physical maintenance. Facilitated transport membranes have particularly high flux and selectivity. We created enzyme-based facilitated transport membranes using isozymes and mutants as immobilized arrays alone and in conjunction with polymeric membranes. The enzyme operates efficiently at the low CO2 concentrations encountered in respiratory gases and can bring CO2 to near ambient levels. CO2 flux is greatly enhanced and selectivities for CO2 over O2 of 200:1 or greater are possible. The enzymes are robust and stable for long periods under a variety of storage and use conditions.

A novel microarray-based approach to simultaneous detection, quantitation, and identification of multiple microorganisms found in water and air supplies is presented. The rapid DNA/RNA probe-based approach avoids time consuming cell cultivation procedures by utilizing the inherent amplification property of rRNA combined with highly sensitive proximal CCD detection. Specifically, optimal DNA probes for simultaneously capturing Escherichia coli and Vibrio proteolyticus were arrayed in a 4 x 4 matrix using a novel microarray printer capable of depositing hundreds of probes within a single well (1cm2) of a conventional 96 well microtiter plate. Following hybridization of a 16S rRNA microorganism mixture to the DNA microarray, detection, imaging, quantitation and identification was performed within seconds employing the complimentary PC-based proximal CCD detector/imager.