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In this study, formation behaviors of soot in laminar diffusion flames of diesel fuel with fatty acid or fatty acid methyl ester (FAME) were investigated. Oleic acid and oleic acid methyl ester were selected as fatty acid and fatty acid methyl ester. Combustion gas emitted from the laminar diffusion flame was sampled, and PM composition in the gas was analyzed. Laser induced incandescence (LII) and laser induced fluorescence (LIF) techniques were applied to measure soot and polycyclic aromatic hydrocarbon (PAH) distributions in the laminar diffusion flames. As the results, soot emission and soot incandescence distributions were decreased by the addition of fatty acid or fatty acid methyl ester. Moreover, PAH concentration in the closed flame became high by addition of fatty acid or fatty acid methyl ester.