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A measurement technique is described which permits quantitative, multi-point, in-cylinder fluid composition measurements in an internal combustion engine. The technique is based on spontaneous Raman scattering with broadband signal collection, and provides simultaneous determination of the mole fractions of H2O, CO2, N2, O2, and fuel (C3H8). Calibration and data reduction procedures which largely eliminate interference between the signals from the various species are discussed. Mean mole fractions obtained in an optical access engine from the beginning of the intake stroke to the end of the compression stroke are reported. The mean mole fractions of the individual species are used to obtain independent estimates of the mean mole fraction of burnt residual gases, which, in turn, are used to evaluate the accuracy and consistency of the data. An over-prediction of the CO2 mole fraction in hot exhaust gases is found to be the most significant shortcoming of the technique.