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As a part of CEEF, a hydro-sphere experiment system has been installed in the air-tight hydro-sphere chamber built in fiscal 1997. Environmental factors in the chamber such as air composition, temperature and humidity could be fully controlled from 360 to 2000ppmCO2, from 20 to 30°C and 70%RH respectively using air composite adjusting system and air-conditioner. In this chamber having 640m3, three aquariums having 20m3 volume each have been installed. One of those aquarium will be used for the sea water buffer, the other two will be used for breeding living organisms such as small animals and macro-algae. A water reclamation system also has been installed in addition to those aquariums, and sea water is to be circulated at the rate of 20m3/3days between those aquariums and this reclamation system in order to realize the simulated sea water eco-system. The water reclamation system is composed of a membrane filter and bio-filter.