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Port fuel injection - PFI - engines are widely available and the calibrations of fuel models are well defined. Modern flex-fuel engines are designed to work with different fuels and a mix of them. However, Brazil’s ethanol is composed of anhydrous Ethanol mixed with 8% distilled water, making it distinct in relation of composition. In a PFI, the fuel mass is mixed to the air mass before entering the combustion chamber in order to keep the mixture most uniform as possible. In this process, heat exchange and partial pressure variation are present and this may lead to volumetric efficiency modification. However, measurement errors may be introduced from the indirect measurement method if it does not considers water content and combustion efficiency to determine volumetric efficiency.