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This study focuses on deposit formation in a diesel engine fueled with straight vegetable oil (SVO) and its effects on engine performance and exhaust emissions. First, two-dimensional thickness distributions of the carbon deposits on the cylinder head were measured by a laser displacement meter after continuous engine operation on gas oil blended with SVO at 25%. The obtained results show that the carbon deposit thickness rapidly increases with SVO-blended fuel and reaches a higher level than with just gas oil. Second, the effects of fuel injector deposits on engine performance and emissions were examined. A small diesel engine was continuously operated by alternating between SVO and gas oil. Gas oil was injected for 1 hour before and after 6 hours of SVO operation to prevent the accumulation of SVO deposits inside the nozzle holes, and the process was repeated.