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A comparative injector test was performed with a direct injection turbocharged, intercooled tractor diesel engine, fueled with a vegetable oil pressed from mustard seeds. The un-esterized mustard seed oil (MSO) was cleaned by simply letting it stand and clear. Engine operation, performance and exhaust emissions were investigated using two-spring injectors as the injection valves. Two injector tips with different orifice diameters were tested. Injection timing was first optimized. Thereafter, performance and exhaust emissions were measured in the large load-speed envelope. The results showed that some benefits were obtained with two-stage injection, particularly at idle, but also at low loads at intermediate speed. The wet exhaust content of oxides of nitrogen decreased at idle, while the CO content and some species of HCs remained almost constant. The NOx emissions also decreased at low loads at intermediate speed.