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Bioprocessing of human fecal wastes may be an important means for recycling of crop nutrients within a closed Advanced Life Support System. The objectives of this study were to determine the levels of key crop nutrients that can be extracted from human feces that had been autoclave sterilized vs. those that had not. When compared with inedible ALS grown wheat residues, the contribution of feces, which has an ash content 13% to the total potential, recoverable minerals may be small. This paper discusses results from bioreactor runs obtained using continuous stirred tank reactors with an 8 day batch culture of autoclaved or not autoclaved feces. The results suggest that feces should not be autoclaved if mineral recovery is desired. Biodegradation of feces ranged from 27 to 39% in 8 days, with 67 to 79% reduction in soluble total organic carbon (TOC) and concomitant production of carbon dioxide (CO2).

Bioreactor technology for bioprocessing graywater solutions in microgravity is under development by NASA at Johnson Space Center and at major aerospace companies. Inoculum sources have been inconsistent. Startup and subsequent operation of ground-based bioreactors may have been adversely affected by this inconsistency and/or by inoculation procedures. The goal of the research reported in this paper is to develop an inoculum that will completely biodegrade Igepon T42 soap to carbon dioxide and water under anaerobic, denitrifying conditions and with process conditions set by bioreactor design requirements for microgravity operation. Potential inoculum sources from two habitats within the KSC-ALS breadboard project were developed for potential use. The effects of pH (7.2 vs. 9.0, buffered) on soap degradation by the two inocula was determined in a flask study. Nearly all of the soap was degraded at pH 7.2 while nearly none was degraded at pH 9.0. Both inocula behaved similarly.