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Mixture preparation is a key contributor to both the combustion and emissions in automotive gasoline engines. The air-fuel ratio near the spark plug may have an effect on combustion characteristics since it is related to early flame development. Therefore, cycle resolved measurements of equivalence ratio near the spark plug is particularly important for better understanding of its contribution on combustion and emissions. This paper describes how we determined the in-cylinder equivalence ratio from the measured hydrocarbon concentration near the spark plug using a Fast Response Flame Ionization Detector (FRFID). The procedures established were then applied to a limited range of engine operating conditions, and the cycle resolved equivalence ratio near the spark plug was determined from the measured hydrocarbon concentration.