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A laser-induced fluorescence (LIF) system has been developed to obtain measurements of the instantaneous lubricant film thickness in the piston-cylinder assembly of a firing single-cylinder, direct-injection diesel engine. Measurements were made at top-dead-centre (TDC), mid-stroke and bottom-dead-centre (BDC) position by means of three fibre optic probes inserted into the cylinder liner and mounted flush with its surface. Following extensive repeatability tests, the cycle-averaged lubricant film thickness was estimated for different multi-grade oils as a function of engine speed, load and temperature. The results quantified the dependence of the film thickness ahead, under and behind the piston rings on oil chemistry and viscometric properties, thus confirming the important role of the LIF technique in the development and formulation of new engine oils.