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The present paper aims at developing a general method to estimate integral and microtime scales of turbulent in-cylinder flow field in reciprocating engines. The ensemble average technique was used to compute the integral time scale from the single point time autocorrelation function, whereas the microtime scale, representative of the most rapid changes that occur in the fluctuation, was computed as the intercept of the parabola that matches the autocorrelation function at the origin. Further, the microtime scale was also estimated by spectral analysis through the energy spectral density function of the ensemble turbulent fluctuation and the results obtained by the two methods were compared. The procedures were applied to the tangential component of the instantaneous velocity data collected, at different engine speeds (1,000, 1,500, 2,000 rpm), within a motored d.i. diesel engine equipped with a re-entrant combustion chamber, using the Laser Doppler Anemometry (LDA) technique.