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Technical Paper

Long-Term Storage of Wheat Plants for Light Microscopy

2000-07-10
2000-01-2231
The objectives of these experiments were two fold: 1) to determine if accepted tissue fixation procedures can be adapted for long-term storage on-board the International Space Station (ISS) and 2) to test whether they are suitable for KSC Fixation Tubes (KFTs). Three tissue fixation parameters were tested: time, temperature and sample size.Fixation time:samples are normally fixed from several minutes to several hours, but because of the duration of the UF-1 mission to ISS, the tissue will be in fixative for up to 3 months. Super dwarf wheat tissue samples were fixed in either FAA (a fixative normally used for storage of plant tissues) or a glutaraldehyde-based fixative and stored for 100 days.
Technical Paper

Reliability of Biological Systems Based on CBF Data

1996-07-01
961498
The reliability of biological life support systems, critical for long-duration human space missions, has been questioned. We propose that properly engineered biological components are inherently reliable, and support this view with data from nine years of operation of the CELSS Breadboard Facility (CBF) at Kennedy Space Center. Reliability problems in a bioregenerative life support system will generally be caused by support system failures, they will generally not be catastrophic, and the crew will have ample time to respond. Thus, biological system reliability can be good, and the impact of low component reliability would generally be to increase system cost rather than to risk mission failure.
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