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Technical Paper

Sterilization of Unibed® Multifiltration Media by Gamma Irradiation

1993-07-01
932045
Unibeds®* are multimedia layered sorption beds baselined for use in the Space Station Freedom (SSF) water reclamation system. Unibeds® must be sterilized prior to use to avoid the introduction of bacteria into the water reclamation system when the Unibeds® are routinely changed out. In the past, Unibeds® were autoclaved in an attempt to achieve sterility. Some sorbent media used in the Unibeds® decompose when exposed to high temperatures for extended periods. Although no significant sorbent decomposition occurs during the routine autoclave time of 30 minutes, it is uncertain whether sufficient sterilization temperatures are achieved at the Unibed® core. Gamma irradiation has been evaluated as a practical alternative method to achieve sterility and eliminate possible sorbent thermal degradation. Sorbent media were inoculated with irradiation resistant spores (106 CFU/ML) of bacterium Bacillus pumilus and subsequently exposed to radiation doses of 1.5, 2.0, and 2.5 megarads (Mrad).
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