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The absorption of unburned fuel into the engine cylinder wall oil film has been identified as a source of hydrocarbon (HC) emissions from spark-ignited (SI)engines. While significant work has been done under steady-state operating conditions to quantify the contribution of this mechanism to overall unburned hydrocarbon emissions, little work has focused on cold starting conditions and in situ measurement of the fuel / oil film interaction. The work reported here shows how laser-induced fluorescence (LIF) spectroscopy can be used to make in situ measurements of the absorption of fuel into the cylinder wall oil film of a single cylinder engine. Measurements were made at two points in the engine cycle under cold start conditions. Results indicate that fuel concentration in the oil film reached a maximum of fifty percent (50%) during cold start operation, though fuel was present in the oil film throughout the engine cycle.