Technical Paper
The Influence of a Particulate Trap on the in Vitro Lung Toxicity Response to Continuous Exposure to Diesel Exhaust Emissions
1999-08-02
1999-01-2710
Recent improvement in exhaust after-treatment technologies allows particle removal up to 95% with traps and NOx removal up to 40% with reduction catalyst from diesel exhausts. The efficiency of the technology is mainly based on the rate of regulated pollutant emission reduction but very few information is available in term of health hazard potential which may be related to interactions with several of the hundreds of chemical species present under different phases (liquid, gas, solid). It is therefore necessary to develop useful tools to evaluate the global toxicity of Diesel exhaust. A model of bi-compartimental gas/liquid organotypic culture of lung tissue has been specifically developed and used for continuous in vitro exposure to the exhaust gas of a direct injection 1.9 l turbo-charged engine. Regulated emissions (CO, HC, NOx and particles) were measured in raw exhaust.
