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The methyl esters of soybean oil, known as biodiesel, are receiving increasing attention as renewable fuels for diesel engines. Biodiesel has a high cetane number, and offers the potential of emission reduction. The properties of biodiesel vary depending on its composition, and this may affect engine performance and emissions. In this project, biodiesel fuels were prepared from feedstocks with modified compositions including the methyl esters of a low palmitic soybean oil, a partially transesterified soybean oil, a synthetic blend of saturated esters, and a commonly used methyl soyate. These esters were blended with No. 2 diesel fuel in 20% and 50% concentrations. The blended fuels were then tested in a diesel engine to investigate the effect of biodiesel composition on performance, combustion characteristics, and emissions.