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The first two phases of the Lunar-Mars Life Support Test Project [LMLSTP] involved housing human volunteers in closed chambers that mimic future extraterrestrial life support systems. The Phase I test involved one person living for 15 days in a chamber with wheat as the primary means of air revitalization. The Phase II test involved 4 people living for 30 days in a chamber with physical/chemical air revitalization and waste water recycling. The consequences of closure on microbial ecology and the influence that microbes had on these closed environmental life support systems were determined during both tests. The air, water, and surfaces of each chamber were sampled for microbial content before, during, and after each test. The numbers of microbes on the Phase I habitation chamber surfaces increased with length of occupation.