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A primary concern in creating a water reclamation system for long-duration manned space flight is the control of microbial contamination which can jeopardize water quality, compromise human health, and degrade constituent materials of the system. The microbial ecology facility in the Analytical and Physical Chemistry Branch of the Materials and Processes Laboratory at NASA's Marshall Space Flight Center (MSFC) is addressing this concern by means of experiments investigating the interaction of bacterial species in the development of a biofilm and their response to the introduction of additional species or to disinfection. Both static and recycling water systems are used. In static experiments, varied sequence and timing of species introduction in binary bacterial biofilms on 316L stainless steel elucidate the mechanisms involved in biofilm formation.

NASA is developing a Water Recovery System (WRS) for Space Station Freedom to reclaim human waste water for reuse by astronauts as hygiene or potable water. A Water Recovery Test (WRT) currently in progress investigates the performance of a prototype of the WRS. Analysis of biofilm accumulation, the potential for microbially influenced corrosion (MIC) in the WRT, and studies of iodine disinfection of biofilm are reported. Analysis of WRT components indicated the presence of organic deposits and biofilms in selected tubing. Water samples from the WRT contained acid-producing and sulfate-reducing organisms implicated in corrosion processes. Corrosion of an aluminum alloy was accelerated in the presence of these water samples; however, stainless steel corrosion rates were not accelerated.

NASA/MSFC is developing a physical/chemical treatment system to reclaim wastewater for reuse on Space Station Freedom (SSF). Integrated testing of hygiene and potable water subsystems assessed the capability to reclaim water to SSF specifications (1)*. The test was conducted from May through July 1990 with a total of 47 days of system test operation. Water samples were analyzed using standard cultural methods employing membrane filtration and spread plate techniques and epifluorescence microscopy. Fatty acid methyl ester (FAME) and biochemical profiles were used for microbial identification. Analysis of waste and product water produced by the subsystems demonstrated the effective reduction of viable microbial populations greater than 8.0E+06 colony forming units (CFU) per 100 mL to an average of 5 CFU/100 mL prior to distribution into storage tanks.