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A comparative study was performed by use of blends of Jatropha oil-diesel fuel and Jatropha oil-kerosene in order to investigate the feasibility of direct utilization of Jatropha oil in a DI diesel engine. Experimental results at low load demonstrated that mixing 60 vol.% of Jatropha oil into both diesel fuel and kerosene gave less impact on indicated thermal efficiency, whereas further increase of Jatropha oil deteriorated it. Jatropha oil-kerosene decreased particulate matter compared to Jatropha oil-diesel fuel, although particulate matter increased with the increase of Jatropha oil fraction. At partial load where double injection was applied, mixing 80 vol.% of Jatropha oil gave no significant impact on indicated thermal efficiency, exhaust gas emissions and particulate matter and no significant difference was observed between diesel fuel blends and kerosene blends.