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Relative motion between the brain and skull may explain many types of brain injury such as intracerebral hematomas due to bridging veins rupture [1] and cerebral contusions. However, no experimental methods have been developed to measure the magnitude of this motion. Consequently, relative motion between the brain and skull predicted by analytical tools has never been validated. In this study, radio opaque markers were placed in the skull and neutral density markers were placed in the brain in two vertical columns in the occipitoparietal and temporoparietal regions. A bi-planar, high-speed x-ray system was used to track the motion of these markers. Due to limitations in current technology to record the x-ray image on high-speed video cameras, only low- speed (﹤ 4m/s) impact data were available.