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A poroelastic characterization of open-cell porous materials using an impedance tube is proposed in this paper. Commonly, porous materials are modeled using Biot’s theory. However, this theory requires several parameters which can be difficult to obtain by different methods (direct, indirect or inverse measurements). The proposed method retrieves all the Biot’s parameters with one absorption measurement in an impedance tube for isotropic poroelastic materials following the Johnson-Champoux-Allard’s model (for the fluid phase). The sample is a cylinder bonded to the rigid termination of the tube with a diameter smaller than the tube’s one. In that case, a lateral air gap is voluntary induced to prevent lateral clamping. Using this setup, the absorption curve exhibits a characteristic elastic resonance (quarter wavelength resonance) and the repeatability is ensured by controlling boundary and mounting conditions.