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Whole-body vibration (WBV) is associated with several adverse health and safety outcomes including low-back pain (LBP) and driver fatigue. The objective of this study was to evaluate the efficacy of three commercially-available air-suspension truck seats for reducing truck drivers’ exposures to WBV. Seventeen truck drivers operating over a standardized route were recruited for this study and three commercially-available air suspension seats were evaluated. The predominant, z-axis average weighted vibration (Aw) and Vibration Dose Values (VDV) were calculated and normalized to represent eight hours of truck operation. In addition, the Seat Effective Amplitude Transmissibility (SEAT), the ratio of the seat-measured vibration divided by the floor-measured vibration, was compared across the three seats. One seat had significantly higher on-road WBV exposures whereas there were no differences across seats in off-road WBV exposures.