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Anti-wear additives are mostly required to improve lubricant properties and hence tribological performance. Addition of nanoparticles to lubricant oils reduces friction and thus enhances the lubrication characteristics. The mechanism of friction reduction in friction could be justified by more than one method. In this work, copper oxide nano-material was added to the engine lubricant oil Mobil 1 SAE15W-40SF with 0.1% wt. concentration. Two new engines were used and operated for 1000 hours, where nanolubricant was added to one of them and regular lubricant was used in the other. Twelve samples were taken periodically from each engine. ASTM-D6595 spectrometry standard was used in order to measure the wear particles in the taken oil samples. Further investigation was done by doing more tests to some of the oil samples using Laser Net Fines Analyzer. Results showed an improvement in the friction properties through a reduction in wear rates in the case of using nano-additives.