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A problem for the diesel engine that remains since its invention is injection nozzle hole fouling. More advanced injection systems and more complex fuels, now also including bio-components, have made the problem more intricate. Zinc and biodiesel have often been accused of being a big part of the problem, but is this really the case? In this study, nozzle fouling experiments were performed on a single cylinder engine. The experiments were divided in three parts, the first part studied the influence of zinc neodecanoate concentration on nozzle hole fouling, the second part studied the effect of neodecanoates of zinc, sodium, calcium, copper, and iron on fuel flow loss and in the last part it was examined how RME concentration in zinc neodecanoate contaminated petroleum diesel affected nozzle hole fouling propensity. After completed experiments, the nozzles were cut open and the deposits were analyzed in SEM and with EDX.