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Biodiesel is a potential alternative to Ultra Low Sulfur Diesel (ULSD); however, it often suffers from increased fuel consumption in comparison to ULSD when injection timings and/or pressures are similar. To decrease fuel consumption, increasing biodiesel injection pressure has been found to mitigate the issues associated with its relatively high viscosity and lower energy content. When doing so, the literature indicates decreased emissions, albeit with potentially greater nitrogen oxide (NOx) emissions in contrast to ULSD. In order to better understand the trade-off between fuel consumption and NOx emissions, this study explores the influence of fuel injection pressure on ULSD, Waste Cooking Oil (WCO) biodiesel, and their blends in a single-cylinder compression ignition (CI) engine. In particular, fuel injection pressures and timings for WCO biodiesel and blended fuels are adjusted to attempt to mimic the in-cylinder pressure profile of operation using ULSD.