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The choice of an appropriate material model with parameters derived from testing and proper modeling of stress-strain response during cyclic loading are the critical steps for accurate fatigue-life prediction of complex automotive subsystems. Most materials used in an automotive substructure, like a chassis system, exhibit combined hardening behavior and it is essential to capture this behavior in the CAE model in order to accurately predict the fatigue life. This study illustrates, with examples, the strain-controlled testing of material coupons, and the calculations of material parameters from test data for the combined hardening material model used in the Abaqus solver. Stress-strain response curves and fatigue results from other simpler material models like the isotropic hardening model and the linear material model with Neuber correction are also discussed in light of the respective fatigue theories.