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As a result of research made during EuroBioRef, five alternative bio based diesel fuels have been produced and tested. The fuels consisted of three different products made from castor oil: Esterol A, Esterol Lot BP093 and Methyl-UCT. The two remaining fuels were POM-Methyl 2.8 and 3-Methylheptane. For the test, the fuels were blended with a reference diesel at a 30%vol ratio. The fuels were tested in a euro 4, 1.6L light-duty high-speed road going turbocharged engine with an EGR-system. The engine was configured with standard injectors and standard ECU settings. The tests were performed on an eddy current dynamometer in four different modes. Analysis shows that the NOx level increased slightly for Esterol A, Methyl-UCT and POM-methyl 2.8. It also showed that CO level was higher for POM-Methyl 2.8 and 3-Methylheptane during highest speed and load.