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An experimental study was conducted on the combustion and emissions characteristics of duel fuel sequential combustion (DFSC) mode on a single-cylinder engine, applying port injection of n-heptane combined with in-cylinder direct injection of commercial gasoline, ethanol and n-butyl alcohol, respectively. Three-stage combustion, which consists of low- and high-temperature combustion of premixed n-heptane and high temperature combustion of directly injected gasoline-like fuels were observed. The effects of the premixed ratio and overall heating values per cycle on the combustion characteristics and emissions were investigated. The experimental results show that: with the increasing of premixed ratio and overall heating values per-cycle, the ignition timing of the directly injected fuels advances and the maximum pressure and maximum mass-averaged temperature increase.