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Wall-deposition of soot particles occurs due to the interaction between spray flame and cylinder liner wall/piston surface, which can potentially affect soot morphology after the in-flame formation/oxidation processes and before the exit from engine cylinder. In order to investigate these effects, flame wall impingement was simulated in a constant volume combustion vessel and thermophoretic soot sampling was conducted for Transmission Electron Microscopic analysis. A TEM grid for the sampling was exposed to a single-shot diesel spray flame multiple times and the variation of soot morphology (concentration, primary particle diameter and aggregate gyration radius) among the multiple exposures was compared. Furthermore, a newly designed impingement-type sampler vertically exposed the grid to the spray flame and sampled soot particles under different boundary condition from that of conventionally used skim-type sampler.