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In order to reduce the pollutant emissions (NOx and PM) of diesel engines, the addition of small gaseous fuel amounts or dual mode operation have been proved as potential techniques. This paper is focused on a detailed characterization of the particles emitted from a single cylinder diesel engine when part of the diesel fuel (5 to 20% by energy) is replaced by a gaseous fuel (producer gas, mainly composed by H2, CO, CH4 and inert compounds) coming from biomass steam gasification. The engine was run at constant speed and torque and different EGR rates. Particle samples were collected by means of fiber glass filters placed in a dilution mini-tunnel. Simultaneously, during tests, part of the exhaust gas was conducted to an SMPS to determine the particle size distribution.