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Biodiesel production has been getting global awareness since Petroleum prices are escalating continuously. As biodiesel is gaining considerable demand, standards are vital for its commercialization and market introduction. Feedstocks availability has posed serious challenges, thus the need for non-edible and unexplored feedstocks has risen. In Indian context, Biodiesel is produced using sal seed oil which is potentially available in Indian forest as a non-edible feedstock. The present paper deals with the production optimization using design of experiments and fuel property characterization of Sal biodiesel (sal methyl esters). Transesterification process parameters like catalyst concentration (% w/w), Oil to Methanol molar ratio, reaction time (min) and reaction temperature (°C) were considered the significance factors and the response was taken as the Yield (% w/w). Experiment matrix with several combinations of factors was generated.