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Fossil fuel reserves are being depleted due to increasing energy requirements. One of the solutions is to partly replace fossil fuel by renewable biodiesel fuel. However, the physical properties of biodiesel fuels need to be thoroughly investigated before applying biodiesel or diesel-biodiesel blends in diesel engines, in order to improve the combustion efficiency. This paper presents the experimental study of diesel fuel and biodiesel blends on injection flow characteristics and fuel spray behavior. Seven fuels were tested: diesel fuel, five diesel-biodiesel blends: 10%(B10), 20%(B20), 30%(B30), 40%(B40), 50%(B50), and pure biodiesel(B100) in a diesel engine equipped with a piezo injector. Injection pressures were set at 30-180 MPa for the study of the injection flow characteristics and at 30-150 MPa for the study of spray behavior in non-vaporizing conditions.