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Bioethanol and plant oil-derived biodiesel are generally considered first generation biofuels. More sustainable and cost effective new biofuels are being designed and produced using modern tools of metabolic engineering and synthetic biology. These new microbial fuels have great potential to become viable alternatives and supplements for petroleum-derived liquid transportation fuels. MAN Latin America has worked in cooperation with REG Life Sciences, a North American industrial biotechnological company, to help in the development of high quality fuels for automotive purposes. The aim of this paper is to present the test engine results of a novel microbially produced fatty acid methyl ester (FAME), under the banner of UltraClean™ Diesel, in a Proconve P7 (Euro V) MAN D0834, diesel engine. Described are a comprehensive performance and emissions evaluation as well as an interpretation of the primary fuel properties.

Studies aimed at partial or total use of renewable fuels in diesel engines has increasingly attracted the interest of the automotive industry, in particular due to its characteristics of strength, durability and greater thermal efficiency. The oil esters derived from biological sources, who receive the generic name for biodiesel, is one of the alternatives used internationally. Their physicochemical properties are similar to diesel oil for providing an acceptable dynamic process of preparation and combustion in the engine. This paper presents and evaluates the results of experimental tests carried out in four trucks used exclusively B20 biodiesel (20% biodiesel and 80% petroleum diesel), and one with B100 (pure biodiesel), in real operating conditions.