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The physiological stress and responses involved in last-minute braking situations are studied very little. The purpose of this study was to investigate older and younger drivers' physiological (central and autonomic nervous systems') responses and driving performance in two unexpected driving situations in a driving simulator. The subjects performed the test for two times, one for unexpected event while driving during 70 km/h and another driving during 90 km/h. An unexpected event described as while the lead-vehicle stops unexpectedly the subject vehicle needs to apply last minute braking. Nineteen healthy older (age: 65.6 ± 5.0 years) and nineteen healthy younger (age: 26.3 ± 2.0 years) drivers performed continuously simulated driving tasks with a simultaneous physiological parameters recording of each subject.