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High energy demand and environmental pollution leads to seeking of new, renewable and clean energy as biofuel and biomass. These fuels are abundant in tropical areas and agricultural-economic-based countries. Among various crops which are used for biofuel, Jatropha Curcas L. Oil (JO) is more beneficial and attractive as it is non-edible which is not competitive with food demand. In agricultural sector, the biomass waste especially from rice production such as rice husk is a tremendous resource in Cambodia. The combination of the use of biomass from rice husk (RH) and Jatropha Cake (JC) from the JO production in the gasification can produce more energy for the electricity production especially in the remote and rural area. In previous research, some researchers have been investigated on the use of JO in blending ratio, heated-neat condition and dual fuel combustion of diesel and bio-digested biogas.