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This study presents the combustion and emissions characteristics of Reactivity Controlled Combustion Ignition (RCCI) produced by early port fuel injection (PFI) of low reactivity n-butanol (normal butanol) coupled with in cylinder direct injection (DI) of cottonseed biodiesel in a diesel engine. The combustion and emissions characteristics were investigated at 5.5 bars IMEP at 1400 RPM. The baseline was taken from the combustion and emissions of ULSD #2 which had an ignition delay of 13° CAD or 1.5ms. The PFI of n-butanol and DI of cottonseed biodiesel strategy showed a shorter ignition delay of 12° CAD or 1.45ms, because of the higher CN of biodiesel. The combustion proceeded first by the ignition of the pilot (cottonseed biodiesel) BTDC that produced a premixed combustion phase, followed by the ignition of n-butanol that produced a second spike in heat release at 2° CAD ATDC.