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With increasing use of biofuels in the automotive industry, it has become necessary to evaluate their effects on the properties of polymers used in the fuel delivery systems. In this study, we have considered the effect of biodiesel on the tensile properties of nylon-6, 30% E-glass fiber reinforced nylon-6 and impact-modified nylon-6. The tensile specimens were immersed in 100% biodiesel for up to 7 days before determining their tensile properties. Another set of specimens were immersed in 100% biodiesel under stressed condition and then their tensile properties were determined. The absorption of biodiesel and their effects on tensile modulus, tensile strength and failure strain are reported in this paper.