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Studies aimed at partial or total use of renewable fuels in diesel engines has increasingly attracted the interest of the automotive industry, in particular due to its characteristics of strength, durability and greater thermal efficiency. The oil esters derived from biological sources, who receive the generic name for biodiesel, is one of the alternatives used internationally. Their physicochemical properties are similar to diesel oil for providing an acceptable dynamic process of preparation and combustion in the engine. This paper presents and evaluates the results of experimental tests carried out in four trucks used exclusively B20 biodiesel (20% biodiesel and 80% petroleum diesel), and one with B100 (pure biodiesel), in real operating conditions.