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Particle number measurements during different real world and legislative driving cycles show that catalyst heating, cold and transient engine operation cause increased particle number emissions. In this context the quality of mixture formation as a result of injector characteristics, in-cylinder flow, operation & engine parameters and fuel composition is a major factor. The goal of this paper is to evaluate the influence of different biogenic and alkylate fuels on the gaseous and particle number emission behavior during catalyst heating operation on a single-cylinder DISI engine. The engine is operated with a late ignition timing causing a high exhaust enthalpy flow to heat up the catalyst, a slightly lean global air fuel ratio to avoid high hydrocarbon emissions and a late injection right before the ignition to reduce the coefficient of variance of the indicated mean effective pressure.