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The adequacy of the fuels with the engines has been often a major goal for the oil industry or car manufacturers. As the formulation of fuels becomes more complex, the use of numerical simulation provides an efficient way to understand and analyze the combustion process. These conclusions become increasingly true with the appearance of second generation biofuels. This paper describes a methodology for the representation of fuels and biofuels using a lumping procedure combined with adequate thermodynamic and thermophysical models. This procedure allows computing different thermodynamic and thermophysical properties for simulation purposes in internal combustion engines. The lumping approach involves reducing analytical data to a few pseudo-components characterized by their molecular weight, critical properties and acentric factor.