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The aim of this study is to explore the anomalous variation of thermo-physical properties of aqueous nanofluids. The specific heat of three water-based nanofluids containing silicon dioxide (SiO₂), titanium dioxide (TiO₂), and aluminum oxide (Al₂O₃) nanoparticles were measured using a differential scanning calorimeter (DSC). Measurements were performed over a temperature range of 30°C - 80°C which was chosen to be between melting point and boiling point of water. The experiments were implemented with different sizes of nanoparticles to investigate the effect of the size of nanoparticles on the specific heat of nanofluids. The specific heat of the nanofluids was plotted as a function of the diameter of nanoparticles and the mass concentration of nanoparticles. The results indicate that the specific heat of aqueous nanofluids decreases as the mass concentration of nanoparticles increases from 0.5% to 20%.