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In the present work, the effect of various nanofluids on automotive engine cooling was experimentally studied. Al2O3, TiC, SiC, MWNT (multi-walled nanotube), and SiO2 nanoparticles with average diameter ranging between 1 and 100 nm were mixed with distilled water to form nanofluids. An ultrasonic generator was used to generate uniform particle dispersion in the fluid. A compatibility test was carried out on all nanofluids and it was found that TiC, MWNT, and Si3N4 nanoparticles settled and separated from the fluid within 3 hours after preparation. The engine cooling performance testing setup consisted of an Aprilia SXV 450 engine, the nanofluid cooling loop, a radiator, a fan, etc. Thermocouples and resistance temperature detectors (RTD’s) were attached to the inlet and outlet of the radiator hose to monitor the temperature changes taking place in the cooling system. A flowmeter was attached to the inlet hose of the radiator to monitor the coolant flow rate.