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Prediction of drowsiness based on an objective measure is demanded in machine and vehicle operations, in which human error may cause fatal accidents. Recently, we focused on the pupil which is controlled by the autonomic nervous system, easily and non-invasively observable from the outside of the body. Prior to the large low frequency pupil-diameter fluctuation, which is known to associate with drowsiness, a Gradual Miosis was observed in most subjects. During this miosis period, the subjects were not yet aware of their drowsiness. We have developed a software system which automatically detects the Gradual Miosis in real time.