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A comprehensive research is presented aiming at assessing the ride comfort of subjects seated into road or off-road vehicles. Although many papers and books have appeared in the literature, many issues on ride comfort are still to be understood, in particular, the paper investigates the mutual effects of the posture and the vibration caused mostly from road unevenness. The paper is divided into two parts. In the first part, a mathematical model of a seated subject is validated by means of actual measurements on human subjects riding on a car. Such measurements refer to the accelerations acting at the subject/seat interface (vertical acceleration at the seat cushion and horizontal acceleration at the seat back). A proper dummy is used to derive the seat stiffness and damping.