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At present, biodiesel fuels using natural-origin materials are expanding in share, and there are many different kinds. Biodiesel fuel generates organic acid when it deteriorates, so care is needed when evaluating the influence of the fuel on automotive fuel-line materials. A model biodiesel fuel was designed taking into account deterioration of the fuel and mixing of impurities into it. Durability of automotive fuel-line rubber and plastic materials were evaluated by using the model fuel. From the evaluation results, it was found that fluoroelastomer (hereafter referred to as FKM) and polyacetal resin (hereafter referred to as POM) deteriorate depending on specific fuel properties and deterioration state. In this paper, we report evaluating results of biodiesel fuels on the automotive fuel-line rubber and plastic materials, and the importance of biodiesel fuel property management.